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Its enduring popularity demonstrates how beverages can become closely associated with national identity and seasonal traditions

Br J Nutr 1989

More recently, a study conducted in human colon carcinoma HCT116 cells showed that lithocholic acid (LCA), a secondary bile acid, stimulates IL-8 expression and induces endothelial cell proliferation and tube like formation in the tumour microenvironment, providing the strong evidence for LCA as a tumour promoter in CRC (126)

Bulk RNA sequencing provides cost-effective transcriptome-wide analysis and enables detection of lowly expressed genes that may be missed by single-cell approaches [52, 53]