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Knowing grade differences also reduces safety and handling risks

10.1111/1541-4337.12389 54 GuL.LiuJ.XuD.LuY

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To construct a diploid homozygous strain expressing URA3 as the counter-selectable marker in the BY4743 background, first pGSH1 sequence (0585 bp upstream of the GSH1 ORF) and HS fragments for URA3 were amplified from yeast genomic DNA