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1 Introduction Wounds are characterized by a deficit in skin tissue and can result from physical injuries (such as burns and surgeries), exposure to chemical irritants, or biological agents (such as diseases and bites)

Similar content being viewed by others Introduction The digenean parasitic worms Fasciola hepatica (temperate liver fluke) and Fasciola gigantica (tropical fluke) are responsible for Fasciolosis

The extraction was done in a buffer [1.0 mm ethylenediaminetetraacetic acid (EDTA), 100 mM TrisHCl (pH 7.3), 100 mM -mercaptoethanol, 1.0 mM phenyl methyl sulfonyl fluoride, 0.1 mM protease inhibitor cocktail, 2% glycerol, 25 mM MgCl 2 , 10 mM dipotassium hydrogen phosphate (K 2 HPO 4 )] and centrifuged at 12,000 g for 15 min, 4 C