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doi: 10.1038/s41467-020-15524-1 313 HernandezALKitzAWuCLowtherDERodriguezDMVudattuNet al

The NFS is based on age, BMI, high blood glucose level, platelet count, albumin level, and AST/ALT ratio

The enzymatic reaction was initiated by the addition of NADPH, glutathione reductase, and 5,5-dithio- bis -2-nitrobenzoic acid in buffer containing EDTA (Sigma-Aldrich, Germany)

Quantitative real-time PCR (qRT-PCR) data indicated no change in BAT-specific mRNA encoding Adrb3, Zic1, and the critical, thermogenic, uncoupled protein Ucp1, suggesting that the islet transplantation does not affect energy expenditure and thermogenesis of BAT