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After treatment, cells were immediately analyzed for apoptosis and the cell cycle using a BD FACSCelesta Cell Analyzer with an Annexin V-FITC/PI Apoptosis Detection Kit and PI/RNase Staining Buffer (BD Biosciences, Franklin Lakes, NJ, USA), respectively, according to the manufacturers instructions

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Many studies, especially those conducted over the last decade leading up to 2026, have explored AOD-9604's efficacy in reducing abdominal fat and promoting weight loss in various models