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Subsequently, a 20-g protein aliquot of each sample was mixed with an equal volume of 2 Laemmli sample buffer (Cat # 1,610,737, Bio Rad, California, USA), separated by sodium dodecyl sulfatepolyacrylamide gel electrophoresis, and then conveyed onto a polyvinylidene difluoride membrane

Thus, PCLS offers a robust, cutting-edge platform that can drive significant advances in the study and treatment of lung IRI

However, most studies indicate that consumption of EDs leads to episodes of increased blood pressure and accelerated and increased cardiac contractility

doi: 10.1371/journal.pone.0170450 41 MonePTrimarcoVKansakarUIzzoRSantulliGTrimarcoB