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Cell viability assay For cell viability assay, cells were planted in 96-well plates (34 10 3 per well) after indicated treatment, then incubated with 10% CCK-8 reagent at 37 C for 2 h

The high sensitivity but lower specificity of the HepG2 cell model with hamster S9-mix therefore makes the model useful when a conservative approach to avoid false-negative results is the goal of the test strategy

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