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coli hosts such as XL-1 Blue (Stratagene Cloning Systems, La Jolla, Calif.) cells are transformed with the ligation mixture and spread on culture plates

Physiol Rev (2008) 88:84186

Modulation of Enzyme Activity in Phases I and II of the Detoxification Pathway Phases I and II of the detoxification pathway contain the most important enzyme group involved in xenobiotic biotransformation ( Stable ITCs (such as SFN, AITC, PEITC, and BITC), have been found to inhibit carcinogen activation through the inhibition of CYP enzymes in animal studies and cell lines ( in vitro model of cells expressing specific human CYP isoforms, proved that PEITC yielded chemopreventive effects against nitrosamine-induced carcinogenesis through CYP450 inhibition (69, Numerous stable ITCs and indole derivatives of GLSs are potent inducers of phase II enzymes (80)

However, the potential for a more targeted outcome keeps it in the conversation