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It also upregulates the expression of the gastrointestinal barrier proteins ZO-1 and occludin in MPTP-treated mice, improves intestinal barrier function and gastrointestinal dysfunction, and consequently exerts a neuroprotective effect indirectly via the gut-brain axis ( Another study demonstrated that administration of neohesperidin, the primary active component of citrus fruits (25 and 50 mg/kg, administered for 24 days), significantly alleviated MPTP-induced damage to dopaminergic neurons in the substantia nigra-striatal pathway of PD mice, increased levels of striatal dopamine (DA) and its metabolite DOPAC, and improved motor dysfunction

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Where the maximum activity was observed in group 6, groups 4 and 5 indicated significantly lower and invariably similar values during all periods of investigation (i.e., days 130)