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Splenocytes from -MYC mice and wild-type littermates were isolated as previously described [53] and stained with APC-conjugated rat anti-mouse CD45R/B220 (ref 561880) and PE-conjugated rat anti-mouse CD43 (ref 561857) antibodies, both from BD Biosciences, before flow cytometry analysis with a FACS Canto II flow cytometer (BD Biosciences) employing Kaluza software (Beckman Coulter)

Glutathione supports both Phase I and Phase II liver detoxification, with GSH conjugation constituting a major elimination pathway for drugs, metabolic waste products, and environmental toxins

They found that although the HFD normally increased susceptibility to PTZ-induced seizures, this effect could be prevented by vitamin E supplementation, likely through its strengthening of the hippocampal antioxidant mechanism [149]

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