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2.5 ALT and AST enzyme activity measurement To evaluate the degree of hepatic injury, the serum enzyme activities of key hepatic enzymes, including alanine aminotransferase (ALT) and aspartate aminotransferase (AST) were assessed

The stock solution was equilibrated at 37 C and 5% CO 2 for 24 h, aliquoted, and stored at 20 C for further use

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A shift in mitochondrial substrates and metabolic inflexibility causes DNA mutations, oxidative stress, and apoptosis in PTPMT1 knockout cells