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Malairuang K, Krajang M, Sukna J, Rattanapradit K, Chamsart S (2020) High cell density cultivation of Saccharomyces cerevisiae with intensive multiple sequential batches together with a novel technique of fed-batch at cell level (FBC)

Western blotting Cells were harvested and suspended in Total Lysis Buffer (0.125 M Tris-HCl pH 6.8, 4% SDS and 20% glycerol)

doi: 10.1002/jbt.22266

Qubit, Invitrogen), and monitored with a Qubit 3.0 Fluorometer (Invitrogen, Life Technologies, Carlsbad, CA, United States), yielding a dsDNA concentration in g/mL