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MATERIALS AND METHODS Materials Dulbeccos Modified Eagle Medium (DMEM), penicillin-streptomycin, Phosphate-buffered saline (PBS), 5-(and-6)-chloromethyl-2,7-dichlorodihydrofluorescein diacetate, acetyl ester (CM-H 2 DCFDA), C11-BODIPY 581/591, Hoechst 33342, Halt Protease and Phosphatase Inhibitor Cocktail (100), and Tissue Protein Extraction Reagent (T-PER) were purchased from Thermo Fisher Scientific, Inc

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During T-cell signaling, it must be reduced in order for the TCR/MHCII/CD4 receptor complex to form: a process that requires CD4 dimerization and domain swapping (Cerutti et al., 2014)