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Briefly, the tissues were homogenized in 300 L of methanol containing 0.01% BHT and a mixture of lipid standards using a micro-tip sonicator

Longer duration exposure to these noise levels is more harmful because it does not allow the ears time to rest and recover

it might be a placebo effect, but I'm not complaining

scRNA-seq analysis, cluster identification, and gene marker determination Data of each individual experiment was analyzed according to the following steps: Raw sequencing data was aligned to the mouse reference genome (GRCm39) and quantified using cellranger count (10 Genomics)