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After multiple adjustment, sex (OR 2.25, CI95% 1.20-4.19, p = 0.011), NLR (OR 1.01, CI95% 1.03-1.16, p = 0.002) and Slow activity (OR 2.84, CI95% 1.31-6.23, p = 0.008) remain significantly associated with ED

subtoxic doses that, when combined over time, become toxic

Detection of GSTP1 metabolically labelled with -alkynyl-palmitate using click chemistry Immunoprecipitated GSTP1 was eluted from the agarose beads conjugated to mouse anti-V5 antibody (V5-10), by incubating at 80C for 15 min in 50 mM HEPES, pH 7.4, with 1% SDS

Pre-activated matrices: matrices which have been chemically modified to facilitate the coupling of specific types of ligand