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DNA methyltransferases inhibitors from natural sources

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The aim of the program is to identify which pesticide residues are prevalent in the agro-food items, determining their concentrations, evaluating if these residues are in accordance with Brazilian regulations and, finally, mapping the most relevant pesticides for food safety in Brazil ( The first consolidated report from this program dates back to 2002, in which there were only nine different food types evaluated

Aliquots of 40 g proteins were then fractionated on 612% SDSpolyacrylamide gels and transferred to PVDF membranes, which were then blocked with 0.1% Tween-20 tris-buffered saline (TBST) solution containing 5% nonfat milk or 5% BSA for 1 h at room temperature