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In existing preclinical studies, Xiong and colleagues found that the co-expression of IL-7 and PH20 in GPC3-targeted CAR-T (G3CAR-7 20) demonstrated significant anti-tumor effects in both in vivo and in vitro experiments, making it a potential treatment for hepatocellular carcinoma [262]

In addition, it is also hypothesized that altered gastric physiology may indirectly reduce intrinsic factor levels

Briefly, cells were grown until a density of 58 x 10 5 /ml is reached, harvested by centrifugation, then nuclear extracts were prepared and stored at 80 C

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