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GeneTailor Site-Directed Mutagenesis System (Invitrogen) was used to perform site-directed mutagenesis of the miR-221 binding site in the IGF-1 3'UTR (designated as mut)

The precise regulation of EVs in the physiological processes outlined above underscores their fundamental role in reproductive success

The results indicated that certain regions within the PPI network exhibited significantly enhanced interactions, and mitochondrial-related proteins with significant causal effects on DKA identified in current analyses were primarily concentrated in these regions (Figure 5B)

Over the next years, it will be important to understand the effect of the reduced circulating amino-acids with the novel multi-agonists targeting the glucagon receptor on lean muscle mass (especially in populations with obesity and high risk of sarcopenia) as well as on energy expenditure [66]