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Description

Forde JA, Dale TC (2007) Glycogen synthase kinase 3: a key regulator of cellular fate

doi: 10.1016/j.jemermed.2011.05.023

The supernatant was transferred into a 1.5 mL centrifuge tube, iodoacetic acid was added at a final concentration of 7 mM to alkylate free thiols, the pH was adjusted to 7 - 8 with saturated K 2 CO 3 and the reaction was allowed to proceed for 1 h in the dark at RT