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Known off-target tendencies: Internal LXXFQG and (V/L)XLFQG-like motifs in your target sequence Slow but real cleavage at LEVLFQA and LEVLFQS significant at overnight scale In GST-PreScission preparations, residual contaminating proteases from production can confound diagnosis Thrombin Consensus: LVPRGS (commonly written as the synthetic LVPRGS)

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However, leptin replacement does not completely prevent the neuroendocrine adaptations induced by weight loss 1, 3, 5 , indicating the existence of critical additional, but still unknown, starvation signals

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