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The tumor tissues were fixed in 4% paraformaldehyde, followed by embedded in paraffin wax, deparaffinized with xylene, treated by ethanol gradient dehydration, and then sliced and stained with H&E, TUNEL (dilution of 1:2:50, catalog number: GB1502, Servicebio), and Ki67 (dilution of 1:200, catalog number: GB111499, Servicebio), respectively