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Down syndrome Down syndrome is due to trisomy 21, the most common chromosomal abnormality occurring in humans

Real-Time Reverse Transcriptase-Polymerase Chain Reaction (RT-PCR) step Real-Time PCR was performed on the extracted RNA using one-step Sybr green kits according to manufacture instructions (Sensi FAST SYBR Lo-ROX one-step Kit (code noBIO-74005)

On the other hand, a stability assay followed by actinomycin treatment showed that HuR enhanced KGA (but not GAC) mRNA stability, in opposition to the reduction of both isoforms stability under ELAVL1 suppression