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American College of Chest Physicians and Canadian Thoracic Society 2015 85 : N-acetylcysteine treatment for patients with moderate to severe COPD and a history of 2 or more exacerbations during the previous 2 years. Conclusions COPD is clearly associated with an excessive lung oxidant burden and the rationale for exploring antioxidant therapies in this patient population is strong

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Western blot analysis of LC3B, Beclin-1, a-SMA, and collagen-I expression from lysates of HSC-T6 incubated with control media, DMKG (4 mM), or Lipoic acid (5 mM) in the presence or absence of C646 (10 mM) for 12 h

Centrifuge at 8,000 x g for 10 minutes at 4C