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Isolation of hepatocytes and culture Mouse hepatocytes were isolated from male mice as previously described using a modification of the in situ collagenase (type I, Worthington) perfusion technique of Seglen 62,84

2.6.1.5 Effects of SL on liver fibrosis A team of Chinese researchers reported that SL inhibited HSC activation and hepatic apoptosis and prevented the formation of mallory-denk bodies in the NASH liver when SL was orally administered to the male C57BL/6 mice which were fed a MCD diet for 8 weeks, and the effect was related to alteration of lipid metabolism-related gene expression, activation of the Nrf2 pathway and inhibition of the NF-B signaling pathway in the NASH liver (Ou et al., 2018)

If leaks are found, discard the solution because the sterility may be impaired

Additionally, these ions have the potential to significantly increase the catalytic POD-mimic activity that nanozymes display