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doi: 10.23986/afsci.5732 338

(2019) 9:187206.e116

doi: 10.1016/j.biopha.2024.116585 102 TanWZhangJChenLWangYChenRZhangHet al

The membranes were blocked for 1 h at room temperature with 5% w/v nonfat dry milk solution, followed by incubation with primary antibodies against p53 (1:1,000) and -actin (1:5,000) at 4C overnight