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In principle, vitamin B12 stores in healthy people last for several years, so a deficiency may only become apparent after two to five years

Managing the correct dosage is crucial for achieving optimal results and minimizing potential side effects

Supplements: If you're at risk (e.g., vegetarians, vegans, or individuals with absorption issues), take vitamin B12 supplements as recommended by a healthcare provider

If this occurs: Allow additional time at room temperature (up to 30 minutes total) Very brief, gentle vortexing (35 seconds) as a last resort If aggregation persists, consider re-solubilizing in 10 mM HCl at a slightly lower concentration 6