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Mechanistically, DMSO is a cell-permeable reagent known to bind to various proteins in the cytoplasm or nucleus 8 , which directly (chemically and physically) interrupts their functions 9

Unfortunately, the more palatable binders tend to be less effective, and concerns exist about using aluminium-based products

Accordingly, unlikely proIGF-1Ea, proIGF-1Eb and proIGF-1Ec did not contain any potential glycosylation sites and migrated at the same molecular weight after treatment of HEK293 cells with Tun (Fig

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