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Cells were washed in PBS, resuspended in Thiazole Orange (BD Retic-Count, BD Bioscience) containing 4 M Hoechst 33342 (Invitrogen) for parasite RNA and DNA detection respectively ( 2 , 5 mM glucose, 32 mM HEPES pH7.4, 5 mM KCl, 1 mM MgSO4, 125 mM NaCl), resuspended in 0.3 M of intracellular calcium dye x-Rhod-1 AM (Invitrogen) and incubated in the dark at 37C for 30 min

Keep a food and skin diary for 46 weeks

An increase in the incorporation of omega-3 PUFAs into cell membranes is associated with decreased prostaglandin and leukotriene production and modulation of the inflammatory process [68]

Changes to mucus permeability are often considered in the context of fertility (transport of sperm), however, they are also associated with the transport of STIs