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p=0.003) Discussion Since the first report of the neurotoxicity of the copper chelator cuprizone in Swiss albino mice by Carlton in 1966 (Carlton, 1966) and the subsequent early description of CPZ-induced OL death and demyelination in the superior cerebellar peduncles of ICI mice (Blakemore, 1972), the CPZ-induced demyelination model has been intensively studied and optimized for more than five decades (Vega-Riquer et al., 2019)