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Description

MS Grade Trypsin Protease (Thermo Fisher Scientific) was added at a 1:20 enzyme-to-protein ratio (w/w), and digestion was carried out at 37 C for 16 h

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Enemas are typically used to relieve constipation or to prepare for a procedure looking at the bowel such as a colonoscopy

Bixin There has been an increasing focus on bixin, an FDA-approved natural food colourant from the seeds of the achiote tree ( Bixa orellana L.) native to tropical America [209, 221, 227]