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Computing was supported via the Advanced Research Computing facility, Oxford, the ARCHER UK National Supercomputing Service and JADE (EP/T022205/1) granted via the High-End Computing Consortium for Biomolecular Simulation, (HECBioSim - supported by EPSRC (EP/R029407/1) to P.C.B

Although evidence has shown the presence of XO in the human brain, which catalyzes purine metabolism to UA, the concentration of UA generated in situ in the brain is low

The slides were washed in water and then blocked using 10% goat serum in PBS for 1 h at room temperature before adding primary antibodies diluted in the blocking buffer

It carries copper into cells where copper-dependent enzymes need it, and the GHK fragment itself acts as a signaling peptide that influences gene expression in fibroblasts and other tissue cells