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Around 200 cells from at least 6 images were quantified for each experimental group

Immunofluorescence staining of cultured neurons To determine the effect of lithium on neuronal morphology, neuron cultures were fixed with 4% paraformaldehyde for 30 min at room temperature, permeabilized with 0.25% Triton X-100 in phosphate buffer saline for 10 min, blocked with 5% donkey serum (Jackson Laboratories, PA) for 1 h at room temperature, and then incubated for 16 h at 4C with rabbit anti-microtubule-associated protein (MAP)2 antibody (Millipore, MA)

Once a pharmacy opens a large stock bottle and repackages the tablets into your small amber vial, real-life storage (bathroom moisture, temperature swings) becomes less predictable, which is why pharmacies often set a 1-year use-by date

Knockdown of either SLC7A5 or SLC7A11 led to a modest reduction in KYN uptake, suggesting cooperative involvement of multiple transporters rather than one dominant pathway (Extended Data Fig