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Description

Polymorphic regulation of mitochondrial fission and fusion modifies phenotypes of microglia in neuroinflammation

10.1093/jxb/erq432 35 FanH

Overall, these results point to a mechanism whereby baseline reductions in mitochondrial complex I activity in PD contribute to excessive inflammation through upregulation of inflammasome activity

Materials and methods Cell culture Human acute monocytic leukemia cells, THP1, and the mouse leukemic monocyte cell line, RAW264.7, were cultured using RPMI-1640 medium modified to exclude calcium nitrate and supplemented with 2.05 mM L-Glutamine (Gibco, C11875500BT), 10% fetal bovine serum (FBS) (ExCell, FSP500), and a 1% penicillinstreptomycin mixed solution (Abiowell, AWH0529a)