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7AD), MDA-MB-231 cells from a highly aggressive, invasive and poorly differentiated triple-negative breast cancer (TNBC) cell line, were maintained in Dulbeccos modified Eagle media, Nutrient Mixture F12 (DMEM/F-12 media) with foetal bovine serum (5%), glutamine (2 mM), sodium pyruvate (1 mM) and non-essential amino acids (0.02 mM) at 37 C in a humidified atmosphere of 5% CO 2

Lomada D, Liu B, Coghlan L, et al

The optimal dispersions exhibited hydrodynamic particle sizes, polydispersity indices, and zeta potentials ranging from 157.5 to 204.4 nm, 0.231 to 0.255, and -27.8 to -18.8 mV, respectively

In vitro hypoxia impairs beta2-adrenergic receptor signaling in primary rat alveolar epithelial cells