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After blocking in 5% skimmed milk in PBS containing 0.05% Tween 20, membranes were probed with anti-human Lyn monoclonal mouse antibody (Santa Cruz) diluted 1:1000, or anti-human IL-1 sRII mouse antibody, Clone 8.5 32 followed by detection with anti-mouse-IgG-horseradish peroxidase conjugate diluted at 1:5000 in blocking buffer (Stratagene)