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In 2021, Antonia Tnase et al

The abdomen and anterior thigh remain preferred due to ease of access, larger surface area for rotation, and consistent adipose thickness across most body compositions

Next, the Annexin V-FITC/PI double-staining assay revealed that ACE treatment induced cell death in HCT116 and RKO cells in a concentration-dependent manner, which was also confirmed by the calcein/PI double-staining assay (Supplementary Fig

134 In cancer cells, OXPHOS increases reactive oxygen species (ROS) production and leads to oxidative stress, activation of p53 signaling and DNA damage, particularly when combined with chemotherapy or other cancer therapies