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Low cardiac lipolysis reduces mitochondrial fission and prevents lipotoxic heart dysfunction in perilipin 5 mutant mice

While slight weight gain will likely not impact your results, anything more than that can start to increase mass on the abdomen, if not other areas more likely to gain fat due to the presence of existing fat cells like the buttocks, thighs, and back

This verification prevents using wrong compounds in research studies

Also, while the manufacturers of metformin recommend against breastfeeding while taking the drug, data have shown that metformin is excreted into breast milk in small amounts and adverse effects on infant plasma glucose have not been reported in human studies