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Samples were processed as follows: 5-10 min in methanol, 1020 min in acetone (both steps at 20 C), followed by incubation in a graded ethanol series (5 min each in 90% / 60% ethanol at 20 C and 5 min in 30% ethanol at room temperature) and 10 min washing in TBST (TBS buffer [20 mM (w/v) tris(hydroxymethyl)-aminomethane pH 7.6, 0.15 M (w/v) NaCl] + 0.2% Tween 20)

Similar to the intestine this transfer is mediated by MTTP

Epub 2011 May 28