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SKU: 74972774807
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For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents: To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions

Fluorescence microscopy For imaging, yeast strains tagged with fluorescent markers were cultured in nutrient-rich medium (YPD) and were treated with 300 M IAA for 8 h for conditional Vma2 degradation or shifted to nitrogen starvation (SD-N) medium for 2 h