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Reactions were terminated by heating at 98 C for 2 min to inactivate Proteinase K, followed by reduction with 10 mM dithiothreitol at 37 C for 30 min and alkylation with 40 mM iodoacetamide at 37 C for 45 min in the dark

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Individuals experiencing severe deficiency symptoms requiring rapid correction benefit from injection therapys immediate action

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