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inhaled), (iv) the composition of the antioxidant agent itself ( i.e ., solubility, adjuvants, and additives), (v) selection of un-standardized, un-validated, or otherwise soft primary outcome indicators, including outcomes of limited relevance to the mechanism of antioxidant action, and (vi) lack of surrogate markers of oxidative stress to accompany functional outcomes

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In tolerance assay, at 42 h after the end of conditioning regimen, shorter preconvulsive latencies than in healthy (non-diazepam conditioned) mice following isoniazid (800 mg/kg i.p.) (as hallmark of tolerance) were observed if diazepam (5.0 mg/kg i.p.) was again given acutely to mice previously conditioned with diazepam alone (use of picrotoxin 3.0 mg/kg i.p., as convulsant, with acute application of diazepam in previously diazepam conditioned mice did not lead to tolerance hallmark)