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Identified cellular targets through which PKA activation produces a bronchodilator effect include: MLCK: Phosphorylation of myosin light chain kinase (MLCK) by cAMP-dependent protein kinase (PKA) decreases the affinity of the kinase for Ca2+ and calmodulin , resulting in a decrease in MLCK activity

The specific screening procedure is summarized in Figure 1

In preclinical research models, exposure to GHK-Cu has been associated with altered gene expression patterns and modulation of signalling pathways related to extracellular matrix dynamics, oxidative stress response, and cellular turnover