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Western blot and real-time PCR Real-time PCR was performed using a Roche LightCycler 480 detection system (Roche, Basel, Switzerland)

Recently, IFN was used to sensitize HCC cells to ferroptosis by suppressing system x c - via activation of the JAK/STAT signaling pathway, thus providing new insights into the feasibility of using IFN to induce ferroptosis in treating HCC [100]