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RR420A) and CFX096 (Bio-Rad, Hercules, CA, USA) using the following thermocycling conditions: Initial denaturation at 95C for 30 sec, followed by 45 cycles at 95C for 5 sec and 60C for 30 sec for annealing and elongation

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The plates were sealed with AeraSeal films (Merck, A9224) to allow gas exchange in cultures and incubated at 30 C, 225 rpm and 80% relative humidity in a humidity shaker (Minitron, INFORS)