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Mahler D, Ohar J, Barnes C, Moran E, Pendyala S, Crater G

Before staining, the slices were washed in 0.1 M PBS thrice and incubated for 1 h in blocking solution (4% Donkey Serum, 0.3% Triton X-100 in 0.1 M PBS)

The sgRNA targeting sequence and the following PAM sequence are highlighted in yellow and green

Pharmacokinetics: Mild symptoms : Patients exhibiting two or more mild symptoms of organophosphate poisoning (Table 1) : Administer one DuoDote injection intramuscularly into the lateral thigh muscle or buttocks (holding the injector for 10 seconds after it fires)