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In nanoparticle manufacture and quality analysis, generally, to maintain the colloidal stability, an electrical charge at the particle surface suspended in liquid (zeta potential value) of 30 to +30 mV is acceptable, owing to their optimal cationic or anionic, while nanoparticles with 10 to +10 mV are considered to have a near-neutral charge ( In practice, given the negative charge of CNS endothelial cells, positively charged nanoparticles enable greater cellular uptake and BBB-penetrating activity compared to negatively charged nanoparticles

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