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3-((tert-butyldimethylsilyl)oxy)-N 1 ,N 2 -dimesitylpropane-1,2-diamine, L2 A 100 mL round bottom Schlenk flask was equipped with 13 mL dry DMF

This on-chip reservoir block is created in PDMS using a similar soft lithography process as described above but using a CNC-milled acrylic layer as the master mold from which the PDMS device was replicated

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Then, the synthesized cDNA was subjected to end-repair, phosphorylation, and A base addition according to Illuminas library construction protocol