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Omega-3 PUFAs have been shown to increase mitochondrial content in skeletal muscle as demonstrated by the upregulation of transcription factors, which govern mitochondrial biogenesis, including PGC 1 and NRF1, in the skeletal muscle of mice fed a high-fat diet (60% energy from fat) supplemented with fish oil (3.4% kcal from n-3 PUFAs) for 10 weeks (Lanza et al., 2013)