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Non-solubilized material was removed by centrifugation for 10 min at 4500 g

Since mTOR is located downstream of AMPK and mTOR has been well reported to regulate muscle growth and metabolism 42,43 as well as the eIF4EBP3L as a gatekeeper of mTOR in muscle growth 44 , we next examined the effect of mTOR pathway by measuring the levels of mTOR and eif4ebp3l in the LMNA mutant transgenic fish with or without drug treatment

Anti-Aging and Cellular Health: ALC is also known for its anti-aging benefits

PCRs were performed using qPCR mix according to the manufacturer's instructions in an ABI 7500 PCR system (Applied Biosystems, USA)