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Supplementary material The Supplementary Material for this article can be found online at: Glossary ALB albumin, Anpep: alanyl-aminopeptidase AOD average optical density (IOD area) CREA creatinine CKD chronic kidney disease CUR curtain gas Cys-Gly l-cysteine-l-glycine DAB 3,3-diaminobenzidine ER estrogen receptor alpha ER estrogen receptor beta ESI electron spray ionization GABA gamma-aminobutyric acid GAPDH glyceraldehyde 3-phosphate dehydrogenase Ggt1 gamma-glutamyltransferase 1 GLU glucose GO Gene Ontology GSH-Px glutathione peroxidase GSH/GSSG ratio reduced/oxidized glutathione ratio H&E hematoxylin and eosin HPL Hypericum perforatum L

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Owing to the absence of -12 and -15 desaturase enzymes, which are necessary for forming carbon-carbon double bonds outside the -12 and -15 carbons, these precursors cannot be produced de novo ( Figure 1 ) in livestock, such as chickens, hence, they should be supplemented in the diet (Ngcobo et al., 2021)